MCD diet (#518810) and CDAA diet (#518753) were purchased from Dyets (Bethlehem, PA, USA). These diets each contained 50 g/kg of corn oil and 100 g/kg of hydrogenated vegetable oil (Primex) as lipid sources.

The choline-deficient, L-amino acid-defined (CDAA) dietary model overcomes these problems to study the development of NASH-induced fibrosis, and this model has been demonstrated to mimic human NASH in both mice and rats by sequentially producing steatohepatitis, liver fibrosis and liver cancer without any loss of body weight (Nakae et al. 1990, 1992; Sakaida et al. 1994; Denda et al. 2007). With rats, optimized feeding with the CDAA diet results in rapid progression of fibrosis followed by a rise in ALT, which is a parameter indicating liver injury. In mice, on the other hand, feeding with the CDAA diet results in little or negligible increase in ALT (Kamada et al. 2007), and long-term feeding of 20 weeks or more is required before liver fibrosis is observed (Denda et al. 2002, 2007; Kodama et al. 2009).

--An improved mouse model that rapidly develops fibrosis in non-alcoholic steatohepatitis

Masahiko Matsumoto*, Natsuko Hada? , Yoshiyuki Sakamaki*, Akiko Uno*, Toshihiko Shiga? , Chiaki Tanaka? , Tsuneo Ito? , Asao Katsume? and Masayuki Sudoh?

Int. J. Exp. Path. (2013), 94, 93–103